human intestinal fibroblasts Search Results


human small intestinal fibroblasts  (Innoprot Inc)
93
Innoprot Inc human small intestinal fibroblasts
Human Small Intestinal Fibroblasts, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+intestinal+fibroblasts/pm41916133-86-0-5?v=Innoprot+Inc
Average 93 stars, based on 1 article reviews
human small intestinal fibroblasts - by Bioz Stars, 2026-07
93/100 stars
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human intestinal fibroblasts  (ScienCell)
90
ScienCell human intestinal fibroblasts
Experimental design. <t>HIFs</t> were co‐cultured with colon cancer HCT‐116 cells to induce CAFs. Induced CAFs were irradiated with blue LED light (465 nm, 30 mW/cm 2 , 30 min) and the supernatant was collected. These supernatants were designated CAF‐conditioned medium (CAF‐CM). Cancer cells were incubated with CAF‐CM. LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; HIFs, human <t>intestinal</t> <t>fibroblasts.</t>
Human Intestinal Fibroblasts, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+intestinal+fibroblasts/pmc12211113-28-0-7?v=ScienCell
Average 90 stars, based on 1 article reviews
human intestinal fibroblasts - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
human primary small intestinal fibroblasts hsif  (Conversant Bio)
90
Conversant Bio human primary small intestinal fibroblasts hsif
Experimental design. <t>HIFs</t> were co‐cultured with colon cancer HCT‐116 cells to induce CAFs. Induced CAFs were irradiated with blue LED light (465 nm, 30 mW/cm 2 , 30 min) and the supernatant was collected. These supernatants were designated CAF‐conditioned medium (CAF‐CM). Cancer cells were incubated with CAF‐CM. LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; HIFs, human <t>intestinal</t> <t>fibroblasts.</t>
Human Primary Small Intestinal Fibroblasts Hsif, supplied by Conversant Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+intestinal+fibroblasts/pmc09700356__Supp_Data-37-0-24?v=Conversant+Bio
Average 90 stars, based on 1 article reviews
human primary small intestinal fibroblasts hsif - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
immortalized human intestine fibroblasts  (AcceGen Biotechnology)
N/A
Immortalized Human Intestine Fibroblasts have been developed by immortalizing primary Human Intestine Fibroblasts with SV40 Large T antigen. Each vial of Immortalized Human Intestine Fibroblasts contains more than 500.000 viable cells.
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human intestine fibroblasts  (Angio-Proteomie)
N/A
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human intestinal fibroblasts  (iXCells Biotechnologies)
N/A
Human Intestinal Fibroblasts (HIF) - Cryopreserved, 0.5 million cells/vial
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human intestinal fibroblasts  (AcceGen Biotechnology)
N/A
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells
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immortalized human small intestine fibroblasts  (Innoprot Inc)
N/A
Immortalized Human Small Intestine Fibroblasts
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Experimental design. HIFs were co‐cultured with colon cancer HCT‐116 cells to induce CAFs. Induced CAFs were irradiated with blue LED light (465 nm, 30 mW/cm 2 , 30 min) and the supernatant was collected. These supernatants were designated CAF‐conditioned medium (CAF‐CM). Cancer cells were incubated with CAF‐CM. LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; HIFs, human intestinal fibroblasts.

Journal: Annals of Gastroenterological Surgery

Article Title: Light‐emitting diode irradiation targets aerobic glycolysis in cancer‐associated fibroblasts to inhibit metabolic coupling with colon cancer cells

doi: 10.1002/ags3.70004

Figure Lengend Snippet: Experimental design. HIFs were co‐cultured with colon cancer HCT‐116 cells to induce CAFs. Induced CAFs were irradiated with blue LED light (465 nm, 30 mW/cm 2 , 30 min) and the supernatant was collected. These supernatants were designated CAF‐conditioned medium (CAF‐CM). Cancer cells were incubated with CAF‐CM. LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; HIFs, human intestinal fibroblasts.

Article Snippet: Human intestinal fibroblasts (HIFs) were purchased from ScienCell Research Laboratories (Cat. 2920, Carlsbad, CA, USA), and were cultured in complete fibroblast medium (Cat. 2301, ScienCell Research Laboratories).

Techniques: Cell Culture, Irradiation, Incubation

HIFs co‐cultured with tumor cells exhibit a CAF phenotype. After co‐culture with HCT‐116 colon cancer cells, the gene expression levels of the CAF markers ACTA2 and FAP in HIFs were determined by RT‐qPCR (A, B). ACTA2; alpha‐smooth muscle Actin, FAP; fibroblast activation protein, CAF; cancer‐associated fibroblast, RT‐qPCR, reverse transcription‐quantitative PCR. * p < 0.05.

Journal: Annals of Gastroenterological Surgery

Article Title: Light‐emitting diode irradiation targets aerobic glycolysis in cancer‐associated fibroblasts to inhibit metabolic coupling with colon cancer cells

doi: 10.1002/ags3.70004

Figure Lengend Snippet: HIFs co‐cultured with tumor cells exhibit a CAF phenotype. After co‐culture with HCT‐116 colon cancer cells, the gene expression levels of the CAF markers ACTA2 and FAP in HIFs were determined by RT‐qPCR (A, B). ACTA2; alpha‐smooth muscle Actin, FAP; fibroblast activation protein, CAF; cancer‐associated fibroblast, RT‐qPCR, reverse transcription‐quantitative PCR. * p < 0.05.

Article Snippet: Human intestinal fibroblasts (HIFs) were purchased from ScienCell Research Laboratories (Cat. 2920, Carlsbad, CA, USA), and were cultured in complete fibroblast medium (Cat. 2301, ScienCell Research Laboratories).

Techniques: Cell Culture, Co-Culture Assay, Gene Expression, Quantitative RT-PCR, Activation Assay, Reverse Transcription, Real-time Polymerase Chain Reaction

Extracellular lactate secretions by HIFs and CAFs. Lactate concentration in CMs was evaluated by lactate assays. HIF, human intestinal fibroblast; CAF, cancer‐associated fibroblast; CM, conditioned medium; * p < 0.05.

Journal: Annals of Gastroenterological Surgery

Article Title: Light‐emitting diode irradiation targets aerobic glycolysis in cancer‐associated fibroblasts to inhibit metabolic coupling with colon cancer cells

doi: 10.1002/ags3.70004

Figure Lengend Snippet: Extracellular lactate secretions by HIFs and CAFs. Lactate concentration in CMs was evaluated by lactate assays. HIF, human intestinal fibroblast; CAF, cancer‐associated fibroblast; CM, conditioned medium; * p < 0.05.

Article Snippet: Human intestinal fibroblasts (HIFs) were purchased from ScienCell Research Laboratories (Cat. 2920, Carlsbad, CA, USA), and were cultured in complete fibroblast medium (Cat. 2301, ScienCell Research Laboratories).

Techniques: Concentration Assay

Gene expression levels of PFK (A) and MCT4 (B) in LED‐irradiated CAFs determined by RT‐qPCR analysis. Protein levels of MCT4 were evaluated by western bloating (C, D). Gene expression levels of OPN3 in HIF, CAF and LED‐irradiated CAFs determined by RT‐qPCR analysis (E). HIF, human intestinal fibroblast; CAF, cancer‐associated fibroblast; CM, conditioned medium; OPN3, Opsin 3; RT‐qPCR, reverse transcription‐quantitative PCR; PFK, Phosphofructokinase; MCT4, Monocarboxylate transporter 4; LED, light‐emitting diode; * p < 0.05.

Journal: Annals of Gastroenterological Surgery

Article Title: Light‐emitting diode irradiation targets aerobic glycolysis in cancer‐associated fibroblasts to inhibit metabolic coupling with colon cancer cells

doi: 10.1002/ags3.70004

Figure Lengend Snippet: Gene expression levels of PFK (A) and MCT4 (B) in LED‐irradiated CAFs determined by RT‐qPCR analysis. Protein levels of MCT4 were evaluated by western bloating (C, D). Gene expression levels of OPN3 in HIF, CAF and LED‐irradiated CAFs determined by RT‐qPCR analysis (E). HIF, human intestinal fibroblast; CAF, cancer‐associated fibroblast; CM, conditioned medium; OPN3, Opsin 3; RT‐qPCR, reverse transcription‐quantitative PCR; PFK, Phosphofructokinase; MCT4, Monocarboxylate transporter 4; LED, light‐emitting diode; * p < 0.05.

Article Snippet: Human intestinal fibroblasts (HIFs) were purchased from ScienCell Research Laboratories (Cat. 2920, Carlsbad, CA, USA), and were cultured in complete fibroblast medium (Cat. 2301, ScienCell Research Laboratories).

Techniques: Gene Expression, Irradiation, Quantitative RT-PCR, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction

Proposed mechanism. LED treatment inhibits aerobic glycolytic metabolism in CAFs to suppress tumor‐stroma metabolic coupling. Schematic representation of LED hindrance of the metabolic coupling between colon cancer cells and CAFs by targeting aerobic glycolysis in CAFs. HIF, human intestinal fibroblast; LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; F‐6‐P, fructose l‐6‐phosphate; F‐1,6‐BP, fructose l‐1,6‐bisphosphate; PFK, Phosphofructokinase; MCT4, Monocarboxylate transporter 4; TCA cycle, tricarboxylic acid cycle.

Journal: Annals of Gastroenterological Surgery

Article Title: Light‐emitting diode irradiation targets aerobic glycolysis in cancer‐associated fibroblasts to inhibit metabolic coupling with colon cancer cells

doi: 10.1002/ags3.70004

Figure Lengend Snippet: Proposed mechanism. LED treatment inhibits aerobic glycolytic metabolism in CAFs to suppress tumor‐stroma metabolic coupling. Schematic representation of LED hindrance of the metabolic coupling between colon cancer cells and CAFs by targeting aerobic glycolysis in CAFs. HIF, human intestinal fibroblast; LED, light‐emitting diode; CAF, cancer‐associated fibroblast; CM, conditioned medium; F‐6‐P, fructose l‐6‐phosphate; F‐1,6‐BP, fructose l‐1,6‐bisphosphate; PFK, Phosphofructokinase; MCT4, Monocarboxylate transporter 4; TCA cycle, tricarboxylic acid cycle.

Article Snippet: Human intestinal fibroblasts (HIFs) were purchased from ScienCell Research Laboratories (Cat. 2920, Carlsbad, CA, USA), and were cultured in complete fibroblast medium (Cat. 2301, ScienCell Research Laboratories).

Techniques: